Application

Research CategorySignaling

Detect Syk phosphorylated on tyrosine 323 using this rabbit polyclonal Anti-phospho-Syk (Tyr323), Alexa Fluor^® 488 Conjugate, Cat. No. ABF1207-AF488, validated for use in Immunocytochemistry.

The unconjugated antibody (Cat. No. ABF1207) is also available for Western blotting application.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tyrosine-protein kinase Syk (EC 2.7.10.2; UniProt P43405; also known as p72-Syk, p72Syk, Spleen tyrosine kinase) is encoded by the SYK gene (Gene ID 6850) in human. Spleen tyrosine kinase (Syk) is a non-receptor tyrosine kinase highly expressed by haematopoietic cells. Syk plays a crucial role not only in adaptive immune receptor signalling, but also in cellular adhesion, innate immune recognition, osteoclast maturation, platelet activation and vascular development. Syk adopts an autoinhibited conformation in its inactive state and is activated upon binding immunoreceptor tyrosine-based activation motifs (ITAMs) and phosphorylation of tyrosine residues in the two linker regions (a.a. 108-160 and 260-370) between SH1 (a.a. 15-107) and SH2 (a.a. 168-259), and between SH2 and the kinase domain (a.a. 371-631). Initial ITAM binding triggers rapid activation of Syk, whereas subsequent phosphorylation leads to prolonged activation. In additon, Syk can phosphorylate ITAMs and autophosphorylate its own link region tyrosines, providing a positive feedback loop following initial ITAM-mediated Syk activation. Syk also mediates signalling of receptors that do not contain an ITAM, including integrins and C-type lectins, via interaction with ITAM-containing adaptors, such as DAP12 (TYROBP) and the FcR gamma-chain (FcRgamma).

Immunogen

Epitope: domain of:

KLH-conjugated linear peptide corresponding to a sequence surrounding phosphorylated Tyr323 of human Syk.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified.

Purified rabbit polyclonal antibody Alexa Fluor^® 488 conjugate in PBS with 1.5% BSA and 0.05% sodium azide.

Quality

Evaluated by Immunocytochemistry in Jurkat cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Syk Tyr323 phosphorylation induction in hydrogen peroxide-treated Jurkat cells.

Specificity

Target phosphotyrosine residue coresponds to pTyr323 of human Syk spliced isoform 1 and pTyr300 of human Syk spliced isoform 2. Sequence surrounding the target tyrosine is not well conserved in mouse and rat Syk.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~72/69 kDa observed. 72.07/69.51 kDa (human Syk isoform 1/2) calculated.